KINETICS AND MECHANISMS OF RELEASE OF SERUM-PROTEINS OF INTOXICATED RAT LIVERS IMPLANTED INTO GUINEA-PIGS

We examined the organic chemistry, kinetics, and mechanisms of release of rat serum proteins by aseptic rat donor livers (DL) pathologically intoxicated by CCl4, alloxan, or cholera toxin implanted into the cir culatory systems in parallel with the host livers (HL) of guinea pigs (GP). This implant model was biologically functional as hepatocytes of the DL released rat plasma proteins into the host's extracellular com partment (ECC) as monitored in GP serum samples collected during 4 h o f the experiment. Furthermore, rat DL hepatocytes retained an in vivo preimplantation pattern of protein biosynthesis acquired in response t o a regulatory mechanism most likely involving leukocytic endogenous m ediator (LEM), kinins, or platelet activating factor (PAF), and conseq uently secreted the entire spectrum of acute-phase reaction (APR) seru m proteins into the ECC of the host GP. Therefore, the impetus to synt hesize and release APR proteins into GP circulation by intoxicated rat liver originated within and was retained by injured rat hepatocytes. (C) 1997 Elsevier Science Ltd.