Hs. Hyun et al., PGE(2) INHIBITS GLUCOSE-UPTAKE IN ISOLATED VILLOUS EPITHELIAL-CELLS OF THE OVINE SMALL-INTESTINE, Journal of veterinary medicine. Series A, 44(7), 1997, pp. 443-447
This study was designed to clarify whether PGE(2) directly inhibits gl
ucose absorption in villous cells. For this purpose, isolated villous
epithelial cells of the ovine small intestine were used, and the uptak
e of C-14-labelled glucose in the absorptive epithelial cells was meas
ured in the presence and absence of 0.2 mM phlorizin or 10 mu M PGE(2)
. In isolated villous epithelial cells of the small intestine in sheep
, net glucose absorption was reduced by 68.2% in the presence of phlor
izin. This result indicates that most of the glucose in ruminants is a
bsorbed by the specific transporter SGLT1, which is sensitive to phlor
izin. PGE(2) decreased glucose absorption by 26.5% in isolated villous
epithelial cells in sheep, although this glucose absorption did not c
hange in the presence of phlorizin. This result clearly indicates the
PGE(2) inhibits the phlorizin-sensitive glucose absorptive process thr
ough direct action on the villous cells of ruminants.