CD34 COUNTS TO PREDICT THE ADEQUATE COLLECTION OF PERIPHERAL-BLOOD PROGENITOR CELLS

Citation
S. Armitage et al., CD34 COUNTS TO PREDICT THE ADEQUATE COLLECTION OF PERIPHERAL-BLOOD PROGENITOR CELLS, Bone marrow transplantation, 20(7), 1997, pp. 587-591
Citations number
14
Categorie Soggetti
Hematology,Oncology,Immunology,Transplantation
Journal title
ISSN journal
02683369
Volume
20
Issue
7
Year of publication
1997
Pages
587 - 591
Database
ISI
SICI code
0268-3369(1997)20:7<587:CCTPTA>2.0.ZU;2-5
Abstract
An essential prerequisite for successful procurement of sufficient aut ologous peripheral blood progenitor cells (PBPC) for engraftment is th e optimal timing of collection. A number of surrogate markers of perip heral blood progenitor cells were analysed to identify a single test w hich could predict the optimum time to harvest, providing at least 2 x 10(6) CD34(+) cells/kg patient body weight. The study comprised 95 pa tients undergoing varied mobilisation regimens with chemotherapy and G -CSF for both solid tumours and haematological malignancies. One hundr ed and fifty-seven PBPC harvests mere collected. Full blood counts (FB C) and CD34(+) cell enumeration was performed on blood samples taken d uring the mobilisation period and immediately prior to leucapheresis ( pre-harvest). All PBPC collections were assayed for colony-forming cel ls and CD34(+) cells in addition to a FBC. The white cell count on the day of harvest showed only weak correlation with the total number of CD34(+) cells in the collection (r = 0.30). In contrast, the absolute number of circulating CD34(+) cells strongly correlated with the CD34( +) cell and CFU-GM yield of the corresponding apheresis product. Provi ded the mobilisation sample contained greater than or equal to 20 x 10 (6) CD34(+) cells/ml, 94% of single collections, performed the followi ng day, contained greater than or equal to 2 x 10(6) CD34(+) cells/kg.