DIFFERENTIAL EFFECT OF INTESTINAL NEUROPEPTIDES ON INVASION AND MIGRATION OF COLON-CARCINOMA CELLS IN-VITRO (VOL 116, PG 111, 1997)

We investigated the effect of neuropeptides, which are vasoactive inte stinal polypeptide (VIP), substance P, (SP), neuropeptide Y (NPY), neu rokinin A (NKA), somatostatin (SOM), calcitonin gene-related peptide ( CGRP), and leucine-enkephalin (L-ENK), on the invasion of murine Colon 26-L5 adenocarcinoma cells through a reconstituted basement membrane (Matrigel) using a Transwell cell culture chamber assay. VIP, SP, NPY, and L-ENK reduced invasive potential of tumor cells in a concentratio n-dependent manner, whereas SOM, CGRP, and NKA had no effect. Especial ly, VIP showed the most effective in inhibiting tumor invasion, and ac hieved 50% reduction at 10(-6) M. A similar effect by VIP was also obs erved in cell migration to fibronectin. VIP had no effect on the growt h of tumor cells at the concentrations ranging from 10(-10) to 10(-6) M. The suppressed ability of the tumor cell motility by VIP (10(-6) M) was practically recovered by co-treatment with 2,5'-dideoxyadenosine, an adenylate cyclase inhibitor. These results indicate that VIP, amon g the neuropeptides used; could inhibit Matrigel invasion of Colon 26- L5 carcinoma cells through partial suppression of their motility, and the reduction was associated with an intracellular cAMP-mediated pathw ay. (C) 1997 Elsevier Science Ireland Ltd.