PHOSPHORYLATION OF SIC1P BY G(1) CDK REQUIRED FOR ITS DEGRADATION ANDENTRY INTO S-PHASE

G(1) cyclin-dependent kinase (Cdk)-triggered degradation of the S-phas e Cdk inhibitor Sic1p has been implicated in the transition from G(1) to S phase in the cell cycle of budding yeast. A multidimensional elec trospray mass spectrometry technique was used to map G(1) Cdk phosphor ylation sites in Sic1p both in vitro and in vivo. A Sic1p mutant lacki ng three Cdk phosphorylation sites did not serve as a substrate for Cd c34p-dependent ubiquitination in vitro, was stable in vivo, and blocke d DNA replication. Moreover, purified phosphoSic1p was ubiquitinated i n cyclin-depleted G(1) extract, indicating that a primary function of G(1) cyclins is to tag Sic1p for destruction. These data suggest a mol ecular model of how phosphorylation and proteolysis cooperate to bring about the G(1)/S transition in budding yeast.