PREPARATION OF RADIOLABELED CLONIDINE FOR USE IN RADIOIMMUNOASSAY

Authors
BHALLA HL MATHEW R SAMUEL G SIVAPRASAD N
Citation
Hl. Bhalla et al., PREPARATION OF RADIOLABELED CLONIDINE FOR USE IN RADIOIMMUNOASSAY, Journal of radioanalytical and nuclear chemistry, 221(1-2), 1997, pp. 93-95
Citations number
6
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Analytical","Nuclear Sciences & Tecnology
Journal title
Journal of radioanalytical and nuclear chemistry → ACNP
ISSN journal
02365731
Volume
221
Issue
1-2
Year of publication
1997
Pages
93 - 95
Database
ISI
SICI code
0236-5731(1997)221:1-2<93:PORCFU>2.0.ZU;2-0
Abstract
The preparation of stable and immunoreactive radioiodinated clonidine for the development of a radioimmunoassay of clonidine is described Ty rosylmethyl ester (TME) conjugate of carboxylic acid derivative of clo nidine was radioiodinated using chloramineT as the oxidizing agent. Th e radioiodinated clonidine was purified by thin layer chromatography, gel filtration and solvent extraction. Radiochemical purity, specific activity and immunoreactivity were determined. The stability of the la beled compound during storage was tested in various solvents at differ ent temperatures.