CLONING AND CHROMOSOME MAPPING OF THE FELINE GENES P21WAF1 AND P27KIP1

For investigation of the relation of cell cycle regulation with tumori genesis in cats, we carried out molecular cloning of feline p21WAF1 an d p27Kip1 cDNAs and chromosomal mapping of these genes on the cat geno me, The feline p21WAF1 cDNA clone obtained in this study encoded 164 a mino acids (aa) showing 83.5% and 76.8% sequence similarity with those of the human and mouse counterparts, respectively. The cat p27Kip1 cD NA clone isolated here encoded 198 aa, showing sequence similarities o f 93.4% and 90.4% with its human and mouse counterparts, respectively. Using a panel of feline x rodent somatic cell hybrids, the feline CDK N1A (p21WAF1) and CDKN1B (p27Kip1) loci were assigned to feline chromo somes B2 and B4, respectively. Southern-blot analyses of 17 feline spo ntaneous leukemia and lymphoma cases using these cDNAs as probes did n ot reveal any rearrangements in either the p21WAF1 or the p27Kip1 gene . RT-PCR/SSCP (single strand conformation polymorphism) analysis of p2 7Kip1 cDNA did not uncover any amino acid substitutions in the 10 feli ne leukemia and lymphoma cases that were examined. (C) 1997 Elsevier S cience B.V.