A. Habenicht et al., SEQUENCE OF THE NON-PHOSPHORYLATING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM NICOTIANA-PLUMBAGINIFOLIA AND PHYLOGENETIC ORIGIN OF THE GENE FAMILY, Gene, 198(1-2), 1997, pp. 237-243
A cDNA-library has been constructed from Nicotiana plumbaginifolia see
dlings, and the non-phosphorylating glyceraldehyde-3-phosphate dehydro
genase (GapN, EC 1.2.1.9) was isolated by plaque hybridization using t
he cDNA from pea as a heterologous probe. The cDNA comprises the entir
e GapN coding region. A putative polyadenylation signal is identified.
Phylogenetic analysis based on the deduced amino acid sequences revea
led that the GapN gene family represents a separate ancient branch wit
hin the aldehyde dehydrogenase superfamily. It can be shown that the G
apN gene family and other distinct branches of the superfamily have it
s phylogenetic origin before the separation of primary life-forms. Thi
s further demonstrates that already very early in evolution, a broad d
iversification of the aldehyde dehydrogenases led to the formation of
the superfamily. (C) 1997 Elsevier Science B.V.