IDENTIFICATION OF P34(CDC2) KINASE FROM SEA-URCHIN HEMICENTROTUS-PULCHERRIMUS AND ITS INVOLVEMENT IN THE PHOSPHORYLATION OF MYOSIN-II REGULATORY LIGHT-CHAIN IN THE METAPHASE EXTRACT

Authors
KOMATSU S MURATAHORI M TOTSUKAWA G MURAI N FUJIMOTO H MABUCHI I HOSOYA H
Citation
S. Komatsu et al., IDENTIFICATION OF P34(CDC2) KINASE FROM SEA-URCHIN HEMICENTROTUS-PULCHERRIMUS AND ITS INVOLVEMENT IN THE PHOSPHORYLATION OF MYOSIN-II REGULATORY LIGHT-CHAIN IN THE METAPHASE EXTRACT, Gene, 198(1-2), 1997, pp. 359-365
Citations number
35
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
198
Issue
1-2
Year of publication
1997
Pages
359 - 365
Database
ISI
SICI code
0378-1119(1997)198:1-2<359:IOPKFS>2.0.ZU;2-R
Abstract
We present here the nucleotide sequence for a cDNA clone encoding p34( cdc2) from sea urchin, Hemicentrotus pulcherrimus. The obtained cDNA c omprised 301 amino acid residues that contained the PSTAIRE domain to be important for binding to cyclins. Amino acid sequence similarity be tween this clone and other eukaryotic cdc2 sequences averaged approxim ately 72%. Using p13(suc1)-conjugated Sepharose 4B and a selective inh ibitor of p34(cdc2) kinase, butyrolactone I, it was first suggested th at p34(cdc2) kinase is involved in the phosphorylation of MRLC at both MLCK site and two PKC sites. (C) 1997 Elsevier Science B.V.