Kgb. Santos et al., GENOTYPE-SPECIFIC NORMALIZATION OF SOYBEAN SOMATIC EMBRYOGENESIS THROUGH THE USE OF AN ETHYLENE INHIBITOR, Plant cell reports, 16(12), 1997, pp. 859-864
The effect of ethylene on somatic embryogenesis from cotyledons of soy
bean (Glycine max) cultivars 'Bragg', 'IAS-5', and 'RS-7' was studied
through the application of silver nitrate or aminoethoxyvinylglycine.
The addition of these chemicals to the induction medium had no effect
an embryo induction, in spite of aminoethoxyvinylglycine having decrea
sed ethylene production and silver nitrate enhancing it. However, subs
equent histodifferentiation and conversion capacity of somatic embryos
was affected by treatments applied to the induction medium. The effec
ts of ethylene on embryo histodifferentiation and conversion were geno
type-specific. Cultivars 'IAS-5' and 'RS-7' produced high frequencies
of dicotyledonous embryos and had high conversion rates. These were al
so the least affected by alterations in ethylene production. For 'Brag
g', which has a low regeneration capacity, the use of aminoethoxyvinyl
glycine led to a significant improvement in the frequency of normal em
bryo formation as well as in the frequency of conversion into plants.
The results suggest that the use of ethylene inhibitors during the ind
uction process may facilitate plant recovery from soybean genotypes, s
uch as 'Bragg', which have a low regeneration capacity.