TRANSCRIPTIONAL REGULATION OF THE CYDDC OPERON, ENCODING A HETERODIMERIC ABC TRANSPORTER REQUIRED FOR ASSEMBLY OF CYTOCHROME-C AND CYTOCHROME-BD IN ESCHERICHIA-COLI K-12 - REGULATION BY OXYGEN AND ALTERNATIVE ELECTRON-ACCEPTORS

Authors
COOK GM MEMBRILLOHERNANDEZ J POOLE RK
Citation
Gm. Cook et al., TRANSCRIPTIONAL REGULATION OF THE CYDDC OPERON, ENCODING A HETERODIMERIC ABC TRANSPORTER REQUIRED FOR ASSEMBLY OF CYTOCHROME-C AND CYTOCHROME-BD IN ESCHERICHIA-COLI K-12 - REGULATION BY OXYGEN AND ALTERNATIVE ELECTRON-ACCEPTORS, Journal of bacteriology, 179(20), 1997, pp. 6525-6530
Citations number
45
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
179
Issue
20
Year of publication
1997
Pages
6525 - 6530
Database
ISI
SICI code
0021-9193(1997)179:20<6525:TROTCO>2.0.ZU;2-H
Abstract
The expression of the cydDC operon was investigated by using a chromos omal Phi(cydD-lacZ) transcriptional fusion and primer extension analys is. A single transcriptional start site was found for cydD located 68 bp upstream of the translational start site, and Northern blot analysi s confirmed that cydDC is transcribed as a polycistronic message indep endently of the upstream gene trxB. cydDC was highly expressed under a erobic growth conditions and during anaerobic growth with alternative electron accepters. Aerobic expression was independent of ArcA and Fnr , but induction of cydDC by nitrate and nitrite was dependent on NarL and Fnr.