A DERIVATIZING REAGENT-KIT USING A SINGLE COUMARIN FLUOROPHORE

6,7-Dimethoxycoumarins having various reactive groups such as bromoace tyl-, hydrazinocarbonyl- and chlorocarbonyl-groups at 3-position on co umarin ring were synthesized for the construction of a fluorescent der ivatizing reagent-kit for carboxylic acids, aldehyde/ketone, alcohol, phenol and amines. These reagents were evaluated from their reactiviti es with model compounds and fluorescent characteristics of products ob tained. Of the reagents, 3-bromoacetyl- and 3-chlorocarbonyl-6,7-dimet hoxycoumarins were found to afford highly fluorescent products with ab ove 0.8 in quantum yields for carboxylic acids, alcohol and aliphatic amines. It was found that the construction of reagent-kit by using a s ingle fluorophore selected after consideration of its emission mechani sm can be significant and useful as an approach for the efficient reag ent development.