PURIFICATION AND CHARACTERIZATION OF TRANSCRIPTION FACTOR IIIA FROM HIGHER-PLANTS

Authors
WYSZKO E BARCISZEWSKA M
Citation
E. Wyszko et M. Barciszewska, PURIFICATION AND CHARACTERIZATION OF TRANSCRIPTION FACTOR IIIA FROM HIGHER-PLANTS, European journal of biochemistry, 249(1), 1997, pp. 107-112
Citations number
55
Categorie Soggetti
Biology
Journal title
European journal of biochemistry → ACNP
ISSN journal
00142956
Volume
249
Issue
1
Year of publication
1997
Pages
107 - 112
Database
ISI
SICI code
0014-2956(1997)249:1<107:PACOTF>2.0.ZU;2-D
Abstract
Transcription factor IIIA (TF IIIA) binds and specifically activates t ranscription of eukaryotic 5S rRNA genes. It also forms a 7S ribonucle oprotein complex with mature 5S rRNA, Here, we describe the purificati on and properties of pTF IIIA from higher plants. The purified protein from tulip (Tulipa whittalii) has a molecular mass of about 40 kDa an d also binds 5S rRNA and 5S rRNA genes. pTF IIIA also facilitates the transcription of a 5S rRNA gene in a HeLa cell extract.