UP-REGULATION OF NM23 NDP KINASE EXPRESSION IN REGENERATING HEPATOCYTES/

We analyzed the kinetics and subcellular localization of nm23-M1 and n m23-M2 in in vivo proliferating mouse liver cells after partial hepate ctomy. Along with the regenerative growth rate measured by mitotic ind ex, the expression of both nm23-M1 and nm23-M2 was altered with distin ct kinetics. Among the subcellular fractions of regenerating hepatocyt es, the increase in the quantity of nm23-M1 and nm23-M2 was the most d ominant in the nuclear fractions. In in vitro proliferating human hepa tocellular carcinoma cells (PLC/PRF/5) stimulated by hepatocyte growth factor, both nm23-H1 and nm23-H2 were dominantly accumulated around n uclear membranes.