CHARACTERIZATION AND PURIFICATION OF ANGIOGENIC FACTOR DERIVED FROM HIGHLY LIVER METASTATIC COLON-CANCER CELLS

We investigated the effects of serum-free conditioned medium (SFCM) of colon cancer cells on human umbilical vein endothelial cells (HUVECs) . SFCM of highly liver metastatic cell line (LM-HS) strongly enhanced proliferation, migration and tube formation of HUVECs. Moreover, it wa s suggested that a main angiogenic factor derived from LM-H5 may be VE GF based on HPLC, SDS-PAGE analysis and neutralizing experiment using anti-growth factor antibodies. These findings suggest that angiogenesi s is related to the processes of metastasis of colon cancer, and that VEGF synthesized by colon cancer cells acts as a key factor inducing a ngiogenesis.