A. Molist et al., ENTEROCYTIC DIFFERENTIATION CORRELATES WITH CHANGES IN THE FINE-STRUCTURE AND SULFATION OF PERLECAN IN HT29 HUMAN COLON-CARCINOMA CELLS, Biochemical and biophysical research communications, 238(3), 1997, pp. 874-879
Undifferentiated HT29 and differentiated HT29G-human colon carcinoma c
ells have been used to study the changes in proteoglycan production an
d structure associated with enterocytic cell differentiation. Differen
tiated cells incorporate twice as mush sulfate than undifferentiated c
ells when labeled with [S-35]sulfate. Bath cell lines produce a hepara
n sulfate proteoglycan which was purified by ion exchange. The heparan
sulfate proteoglycan from differentiated HT29G- cells as larger and m
ore homogeneous in size than that produced by undifferentiated HT29 ce
lls. No differences in the core protein structure were observed. The d
etailed structural analysis of the heparan sulfate chains revealed tha
t the structure of these chains follows the standard rules for these g
lycosaminoglycans with N-sulfated domains and N-acetylated domains. Th
e main finding was that differentiated HT29G- cells have a degree of h
igher sulfation than HT29 cells. These differences were found to affec
t primarily 6-O-sulfated positions. (C) 1997 Academic Press.