INTERACTION OF LYSOZYME WITH SYNTHETIC ANTILYSOZYME D1.3 ANTIBODY FRAGMENTS STUDIED BY AFFINITY-CHROMATOGRAPHY AND SURFACE-PLASMON RESONANCE

Synthetic antibody fragments of monoclonal anti-lysozyme antibody D1.3 have been tested on binding with hen egg white lysozyme using immunoa ffinity chromatography and surface plasmon resonance. Upon immunoaffin ity chromatography, peptides containing one or two complementarity det ermining regions (CDRs) of D1.3 show interaction with lysozyme. Surfac e plasmon resonance with immobilized CDR peptides showed that this int eraction is not based on the antigen-antibody interaction. Nevertheles s, these peptides could be useful as ligands for the purification of l ysozyme from a mixture of proteins.