MOLECULAR-SIZE DETERMINATIONS OF DNA RESTRICTION FRAGMENTS AND POLYMERASE CHAIN-REACTION PRODUCTS USING CAPILLARY GEL-ELECTROPHORESIS

Commercially available capillary electrophoresis (CE) systems offer ad vantages over traditional slab gel methodologies. The capillary format allows the use of higher voltages (225 V/cm), which results in faster migration, higher resolution and greater efficiency without excessive heating. The ability to automate the system increases the unattended sample analysis throughput. For this study, the CE system was configur ed with a mu PAGE 3% T, 3% C polyacrylamide gel capillary with an effe ctive length of 40 cm and mu PAGE Tris-borate urea buffer system. The analysis of DNA restriction fragments and polymerase chain reaction pr oducts, with an internal standard of Boehringer Mannheim DNA marker XI were completed in less than 70 min. All samples were analyzed at 260 nm. The data establishes automated capillary gel electrophoresis as a high-resolution, reproducible method for analysis of samples under 100 0 base pairs.