GENETIC-ANALYSIS OF NEEDLE PROTEINS IN MARITIME PINE .1. MAPPING DOMINANT AND CODOMINANT PROTEIN MARKERS ASSAYED ON DIPLOID TISSUE, IN A HAPLOID-BASED GENETIC-MAP
C. Plomion et al., GENETIC-ANALYSIS OF NEEDLE PROTEINS IN MARITIME PINE .1. MAPPING DOMINANT AND CODOMINANT PROTEIN MARKERS ASSAYED ON DIPLOID TISSUE, IN A HAPLOID-BASED GENETIC-MAP, Silvae Genetica, 46(2-3), 1997, pp. 161-165
A genetic analysis of proteins was performed in a 3-generation inbred
pedigree of maritime pine (Pinus pinaster (AIT.). Proteins were extrac
ted from needles (2n) and revealed by 2-dimensional gel electrophoresi
s. A total of 17 qualitative variants (presence/absence variations and
position shifts) were observed and conformed to Mendelian inheritance
patterns. These markers were localized in a previously reported genet
ic map based on RAPD markers assayed on megagametophytes (1n). To achi
eve this integration, evenly spaced RAPD markers were genotyped on dip
loid tissue. The internal amino acid sequences of 3 proteins were dete
rmined, and 2 of them corresponded to genes of known function. We disc
uss the use of proteins separated in 2-dimensional gels for constructi
ng a genetic map of expressed genes.