GENETIC-ANALYSIS OF NEEDLE PROTEINS IN MARITIME PINE .1. MAPPING DOMINANT AND CODOMINANT PROTEIN MARKERS ASSAYED ON DIPLOID TISSUE, IN A HAPLOID-BASED GENETIC-MAP

A genetic analysis of proteins was performed in a 3-generation inbred pedigree of maritime pine (Pinus pinaster (AIT.). Proteins were extrac ted from needles (2n) and revealed by 2-dimensional gel electrophoresi s. A total of 17 qualitative variants (presence/absence variations and position shifts) were observed and conformed to Mendelian inheritance patterns. These markers were localized in a previously reported genet ic map based on RAPD markers assayed on megagametophytes (1n). To achi eve this integration, evenly spaced RAPD markers were genotyped on dip loid tissue. The internal amino acid sequences of 3 proteins were dete rmined, and 2 of them corresponded to genes of known function. We disc uss the use of proteins separated in 2-dimensional gels for constructi ng a genetic map of expressed genes.