MOLECULAR-CLONING, CDNA SEQUENCE-ANALYSIS, AND CHROMOSOMAL LOCALIZATION OF MOUSE PKD2

The gene responsible for the second form of autosomal dominant polycys tic kidney disease, PKD2, has recently been identified. We now describ e the cloning, genomic localization, cDNA sequence, and expression ana lysis of its murine homologue, Pkd2. The cloned cDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NB2 and C OOH termini. Pkd2 is highly conserved with 91% identity and 98% simila rity to polycystin-2 at the amino acid level. Pkd2 mRNA is widely expr essed in mouse tissues. Pkd2 maps to mouse Chromosome 5 and is exclude d as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype. (C) 1997 Academic Press.