THE HUMAN TRANSALDOLASE GENE (TALDO1) IS LOCATED ON CHROMOSOME-11 AT P15.4-P15.5

Transaldolase (TAL) is a key enzyme of the pentose phosphate pathway, which is. responsible for generation of reducing equivalents to protec t cellular integrity from reactive oxygen intermediates, While exons 2 and 3 are highly repetitive, the complete TAL-H gene is mapped to a s ingle genomic locus (TALDO1(2)) by several independent approaches. Sou thern blot hybridization of a 827-bp 3' EcoRI fragment of the TAL-H cD NA to human-mouse somatic cell hybrid DNA localized TALDO1 to the p13- ->pter region of chromosome 11. Fluorescence in situ hybridization wit h a 15-kb genomic fragment harboring exons 1 and 2 mapped TALDO1 to 11 p15.4-p15.5. A truncated and mutated segment of TAL-H exon 5 terminati ng with a poly(A) tail was identified in a pseudogene locus (TALDOP1) on chromosome 1. Reverse transcriptase-PCR studies of human-mouse soma tic cell hybrids revealed the presence of the functional TAL-H gene on chromosome 11 and its absence on human chromosome 1, Mapping of radia tion hybrids placed TALDO1 between markers WI-1421 and D11S922 on 11p1 5. (C) 1997 Academic Press.