RAPD analysis was employed to assess genetic variation in named cultiv
ars of garlic (Allium sativum L.), and to examine the relationships be
tween cultivated garlic varieties and the wild progenitor Allium longi
cuspis. Twenty-seven accessions were subjected to RAPD analysis using
26 oligonucleotide primers. Of a total of 292 bands, 63 (21%) were pol
ymorphic. Cluster analysis revealed groupings that in part reflected p
atterns of morphological variation. All bolting forms (including wild
and cultivated) grouped separately from the nonbolting cultivars, A. l
ongicuspis and var. ophioscorodon grouped together, indicating close t
axonomic affinity. Based upon relative levels of variation within diff
erent groups, we suggest potential relationships within the A. sativum
/A. longicuspis complex.