POSITIONING OF 2 ALPHA-SUBUNIT CARBOXY-TERMINAL DOMAINS OF RNA-POLYMERASE AT PROMOTERS BY 2 TRANSCRIPTION FACTORS

Authors
MURAKAMI K OWENS JT BELYAEVA TA MEARES CF BUSBY SJW ISHIHAMA A
Citation
K. Murakami et al., POSITIONING OF 2 ALPHA-SUBUNIT CARBOXY-TERMINAL DOMAINS OF RNA-POLYMERASE AT PROMOTERS BY 2 TRANSCRIPTION FACTORS, Proceedings of the National Academy of Sciences of the United Statesof America, 94(21), 1997, pp. 11274-11278
Citations number
27
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
94
Issue
21
Year of publication
1997
Pages
11274 - 11278
Database
ISI
SICI code
0027-8424(1997)94:21<11274:PO2ACD>2.0.ZU;2-T
Abstract
Interactions between the cAMP receptor protein (CRP) and the carboxy-t erminal regulatory domain (CTD) of Escherichia coli RNA polymerase alp ha subunit were analyzed at promoters carrying tandem DNA sites for CR P binding using a chemical nuclease covalently attached to alpha. Each CRP dimer was found to direct the positioning of one of the two alpha subunit CTDs. Thus, the function of RNA polymerase may be subject to regulation through protein-protein interactions between the two (alpha subunits and two different species of transcription factors.