EXPLORING THE METABOLIC AND GENETIC-CONTROL OF GENE-EXPRESSION ON A GENOMIC SCALE

DNA microarrays containing virtually every gene of Saccharomyces cerev isiae were used to carry out a comprehensive investigation of the temp oral program of gene expression accompanying the metabolic shift from fermentation to respiration. The expression profiles observed for gene s with known metabolic functions pointed to features of the metabolic reprogramming that occur during the diauxic shift, and the expression patterns of many previously uncharacterized genes provided clues to th eir possible functions. The same DNA microarrays were also used to ide ntify genes whose expression was affected by deletion of the transcrip tional co-repressor TUP1 or overexpression of the transcriptional acti vator YAP1. These results demonstrate the feasibility and utility of t his approach to genomewide exploration of gene expression patterns.