SIMPLE METHOD FOR MONITORING ENZYMATIC-REACTIONS BY DIRECT-INJECTION OF THE MEDIUM REACTION IN A GAS-CHROMATOGRAPH EQUIPPED WITH PACKED LINER

Authors
DASILVA FM BASTOS FD OESTREICHER EG PINTO GF JONES J PAIVA LMC
Citation
Fm. Dasilva et al., SIMPLE METHOD FOR MONITORING ENZYMATIC-REACTIONS BY DIRECT-INJECTION OF THE MEDIUM REACTION IN A GAS-CHROMATOGRAPH EQUIPPED WITH PACKED LINER, Biotechnology techniques, 11(10), 1997, pp. 739-741
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
10
Year of publication
1997
Pages
739 - 741
Database
ISI
SICI code
0951-208X(1997)11:10<739:SMFMEB>2.0.ZU;2-1
Abstract
Reduction of sulcatone, catalyzed by Thermoanaerobium brockii alcohol dehydrogenase in Tris/HCl buffer (pH 7.8) using 2-propanol 20% (v/v) a s an NADPH regeneration system could be followed by using a gas chroma tograph equipped with a packed liner that allowed the direct injection of the aqueous medium reaction. The time-course curve obtained by the analytical method here described and that obtained by spectrophotomet ric methods were similar.