PHOSPHATED CYCLODEXTRINS AS NEW ACIDIC CHIRAL ADDITIVES FOR CAPILLARYELECTROPHORESIS

The study describes the use of alpha,beta, and gamma phosphated cyclod extrins (CDs) for chiral separation by capillary electrophoresis (CE). A systematic study was performed to establish the effect of such vari ables as the type and concentration of chiral selectors as well as the pH and ionic strength of buffer and organic modifiers. It was found t hat CDs with acidic character offer high chiral selectivity toward bas ic compounds. These CDs can give chiral recognition features toward mo lecules in their ionized as well as in their undissociated form. The p hosphated cyclodextrins were also found to have adsorptive properties resulting in mixed-migration mechanism, and some guidelines are given to avoid this side effect. A number of racemic drug molecules were use d as model compounds to demonstrate optimization procedures of the com peting parameters in the analysis. (C) 1997 John Wiley & Sons, Inc.