Objectives. The purpose of this study was to identify circumstances in
which biochemical assessments of smoking produce systematically highe
r or lower estimates of smoking than self-reports. A secondary aim was
to evaluate different statistical approaches to analyzing variation i
n validity estimates. Methods. Literature searches and personal inquir
ies identified 26 published reports containing 51 comparisons between
self-reported behavior and biochemical measures. The sensitivity and:s
pecificity of self-reports of smoking were calculated for each study a
s measures of accuracy. Results. Sensitivity ranged from 6% to 100% (m
ean = 87.5%), and specificity ranged from: 33% to 100% (mean = 89.2%).
Interviewer-administered questionnaires, observational studies, repor
ts by adults, and biochemical validation with cotinine-plasma were ass
ociated with higher estimates of sensitivity and specificity. Conclusi
ons. Self-reports of smoking are accurate in most studies. To improve
accuracy, biochemical assessment, preferably with cotinine-plasma, sho
uld be considered in intervention studies and student populations.