I. Sardi et al., THE USE OF RT-NESTED PCR OF PROSTATE-SPECIFIC ANTIGEN TO DETECT HEMATOGENOUS NEOPLASTIC-CELLS IN PATIENTS WITH PROSTATE ADENOCARCINOMA, Journal of molecular medicine, 75(10), 1997, pp. 751-757
Citations number
24
Categorie Soggetti
Medical Laboratory Technology","Genetics & Heredity
The aim of this study was to determine the presence of hematogenous ne
oplastic cells in patients with prostate cancer. We used a reverse tra
nscription (RT) ''nested'' polymerase chain reaction (PCR) of prostate
-specific antigen (PSA) mRNA to detect the presence of circulating tur
ner cells in 52 patients who underwent radical prostatectomy with lymp
hadenectomy. Blood samples were obtained before and after the surgical
manipulation. Seven (13.5%) preoperative samples presented evidence o
f circulating neoplastic cells. All postoperative specimens studied pr
esented a negative result at analysis 24 h after surgical manipulation
. Although we did not find a statistical correlation between the PSA-P
CR results and clinical-histopathological parameters, the presence of
circulating prostate cells was strongly correlated with an elevated Gl
eason score of primary tumor (P<0.01). Thus our data show the positive
effect of surgical treatment in removing the metastases source. The s
ensitive RT-nested PCR assay may play a crucial role in the administra
tion of adjuvant therapy of patients with prostate adenocarcinoma.