COIMMOBILIZATION OF L-ALPHA-GLYCEROPHOSPHATE OXIDASE WITH CATALASE AND ITS APPLICATION FOR THE SYNTHESIS OF DIHYDROXYACETONE PHOSPHATE

Authors
KRAMER L STECKHAN E
Citation
L. Kramer et E. Steckhan, COIMMOBILIZATION OF L-ALPHA-GLYCEROPHOSPHATE OXIDASE WITH CATALASE AND ITS APPLICATION FOR THE SYNTHESIS OF DIHYDROXYACETONE PHOSPHATE, Tetrahedron, 53(43), 1997, pp. 14645-14650
Citations number
13
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron → ACNP
ISSN journal
00404020
Volume
53
Issue
43
Year of publication
1997
Pages
14645 - 14650
Database
ISI
SICI code
0040-4020(1997)53:43<14645:COLOWC>2.0.ZU;2-O
Abstract
The oxidation of L-alpha-glycerophosphate (1) to dihydroxyacetone phos phate (2) is carried out enzymatically under aerobic conditions by L-a lpha-glycerophosphate oxidase coimmobilized with catalase, which serve s for the decomposition of the generated hydrogen peroxide. 2 is trapp ed in an aldolase reaction with D/L-glyceraldehyde (3). The coimmobili zed enzymes show an increased operational stability and can be reused in further transformations without loss of activity. (C) 1997 Elsevier Science Ltd.