STEP-GRADIENT CAPILLARY ELECTROCHROMATOGRAPHY

The analytical benefits of using a step-gradient in; capillary electro chromatography (CEC) are demonstrated. The application of step-gradien t CEC to the analysis of six diuretics of widely differing lipophilici ties was evaluated and shown to result in a marked reduction in the an alysis time and an improvement in the peak shape for later-eluting lip ophilic components. When the step-gradient approach was performed in a n automated mode, the retention time RSD for repeated injections was b elow 1%.