R. Hettich et Hj. Schneider, SUPRAMOLECULAR CHEMISTRY .71. EVIDENCE FOR HYDROLYTIC DNA CLEAVAGE BYLANTHANIDE(III) AND COBALT(III) DERIVATIVES, Perkin transactions. 2, (10), 1997, pp. 2069-2072
Double stranded plasmidic DNA, cleaved by either europium(III) salts o
r by the cobalt(III)-cyclen complex, is religated for the first time w
ithout the use of enzymes, showing hydrolytic mechanisms with these ch
emical nucleases. The same result is observed in the hydrolysis of the
dinucleotide TpT by Eu-III, which is achieved in 25% conversion. The
only products observed by HPLC are 3',5'-cTMP, 3'-TMP, 5'-TMP and thym
idine. The nucleobase thymine itself is not detected (<1%), which demo
nstrates the absence of radical cleavage. The dinucleotide hydrolysis
shows, with [Eu-III] = 5 mM, rate enhancement factors of >10(7), which
even exceed those observed with activated phenyl phosphates.