SUPRAMOLECULAR CHEMISTRY .71. EVIDENCE FOR HYDROLYTIC DNA CLEAVAGE BYLANTHANIDE(III) AND COBALT(III) DERIVATIVES

Citation
R. Hettich et Hj. Schneider, SUPRAMOLECULAR CHEMISTRY .71. EVIDENCE FOR HYDROLYTIC DNA CLEAVAGE BYLANTHANIDE(III) AND COBALT(III) DERIVATIVES, Perkin transactions. 2, (10), 1997, pp. 2069-2072
Citations number
33
Categorie Soggetti
Chemistry Physical","Chemistry Inorganic & Nuclear
Journal title
ISSN journal
03009580
Issue
10
Year of publication
1997
Pages
2069 - 2072
Database
ISI
SICI code
0300-9580(1997):10<2069:SC.EFH>2.0.ZU;2-V
Abstract
Double stranded plasmidic DNA, cleaved by either europium(III) salts o r by the cobalt(III)-cyclen complex, is religated for the first time w ithout the use of enzymes, showing hydrolytic mechanisms with these ch emical nucleases. The same result is observed in the hydrolysis of the dinucleotide TpT by Eu-III, which is achieved in 25% conversion. The only products observed by HPLC are 3',5'-cTMP, 3'-TMP, 5'-TMP and thym idine. The nucleobase thymine itself is not detected (<1%), which demo nstrates the absence of radical cleavage. The dinucleotide hydrolysis shows, with [Eu-III] = 5 mM, rate enhancement factors of >10(7), which even exceed those observed with activated phenyl phosphates.