ESTIMATION OF ANEUPLOIDY FOR CHROMOSOME-3, CHROMOSOME-7, CHROMOSOME-16, CHROMOSOME-X AND CHROMOSOME-Y IN SPERMATOZOA FROM 10 NORMOSPERMIC MEN USING FLUORESCENCE IN-SITU HYBRIDIZATION

Fluorescence in-situ hybridization (FISH) is a fast and efficient meth od of estimating aneuploidy in human spermatozoa. In this study, we ha ve estimated baseline disomy frequencies in spermatozoa from a group o f 10 normospermic men, using stringent scoring criteria. A triple-prob e FISH procedure was used for chromosomes 3, X and Y, while a double-p robe FISH method was used for chromosomes 7 and 16. A total of 101 273 spermatozoa were scored for chromosomes 3, X and Y, resulting in 97.8 3% haploidy (3X or 3Y), 0.39% disomy (33X, 33Y, 3XX, 3YY or 3XY) and 0 .85% diploidy (33XX, 33YY or 33XY). A total of 100 760 spermatozoa wer e scored for chromosomes 7 and 16, giving 98.9% haploidy (716), 0.11% disomy (7716 or 71616) and 0.27% diploidy (771616). Disomy frequencies for individual chromosomes differed (chromosome 3, 0.20%; chromosome 7, 0.05%, chromosome 16, 0.06%; X+Y, 0.19%). The frequency of disomy 3 was significantly higher than disomy 7 (P = 0.019) and disomy 16 (P = 0.022), while the frequency of sex chromosome disomy was significantl y higher than disomy 7 (P = 0.0058) and disomy 16 (P = 0.0067), but no t disomy 3 (P = 0.73). The disomy and diploidy (0.27-0.35%) estimates obtained for this normospermic population were generally low and were similar to other recent reports.