T. Murata et al., EXPRESSION OF CALCIUM-BINDING PROTEIN REGUCALCIN MESSENGER-RNA IN THECLONED HUMAN HEPATOMA-CELLS (HEPG2) - STIMULATION BY INSULIN, Molecular and cellular biochemistry, 175(1-2), 1997, pp. 163-168
The expression of hepatic Ca2+-binding protein repucalcin in the clone
d human hepatoma cells (HepG2) was investigated, The change in regucal
cin mRNA levels was analyzed by Northern blotting using rat liver regu
calcin complementary DNA (0.9 kb of open reading frame). Regucalcin mR
NA was expressed in HepG2 cells, although the mRNA was markedly expres
sed in normal rat liver. Moreover, regucalcin protein in HepG2 cells w
as detected by Western blot analysis using a polyclonal rabbit anti-re
gucalcin antibody. Regucalcin mRNA expression in HepG2 cells was clear
ly stimulated by the culture with insulin (10(-8) M) of the effective
concentration. Regucalcin protein in HepG2 cells was also increased by
the treatment of insulin (10(-8) M). The present results demonstrate
that regucalcin is expressed in the transformed HepG2 cells, and that
the expression is stimulated by insulin.