ISOLATION AND CHARACTERIZATION OF DEFATTED CANOLA-MEAL PROTEIN

Protein was extracted and isolated from canola oil processing waste. C anola is an increasingly important oilseed crop worldwide. More than 9 9% of protein was extracted from crude commercial hexane defatted cano la meal when a 5% w/v suspension in 0.4% w/v NaOH was agitated for 60 min at room temperature in baffled flasks on an orbital shaker at 180- 200 rpm. Protein recovery was 87.5% upon precipitation with acetic aci d. The major proteins in canola seed were glutelins (91.8% soluble in 0.4% w/v NaOH) and globulins (50.9% soluble in 5% w/v NaCl) as measure d by separate extractions. Canola proteins had poor solubility between pH 2 and 10 for all dispersion solutions. Solubility of protein isola tes was 60% or less. Extraction and precipitation procedure did not pr oduce lysinoalanine. Amino acid profile of isolates indicated high nut ritional quality for use in products for 10-12-year-olds and adults.