AMPLIFICATION AND FUSION OF LONG FRAGMENTS OF HEPATITIS-C VIRUS GENOME

Authors
WANG LF RADKOWSKI M VARGAS H RAKELA J LASKUS T
Citation
Lf. Wang et al., AMPLIFICATION AND FUSION OF LONG FRAGMENTS OF HEPATITIS-C VIRUS GENOME, Journal of virological methods, 68(2), 1997, pp. 217-223
Citations number
12
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
68
Issue
2
Year of publication
1997
Pages
217 - 223
Database
ISI
SICI code
0166-0934(1997)68:2<217:AAFOLF>2.0.ZU;2-L
Abstract
The 'long PCR' was used for amplification of hepatitis C virus (HCV) s ubgenomic fragments from liver. After testing several commercially ava ilable systems, it was found that Tth as the major enzyme is superior to using Tag. Employing a mixture of Tth and Vent polymerase (rTth pol ymerase, XL, Perkin Elmer) it was possible to amplify 4.6-kb and 9-kb fragments from biological samples containing as little as 10(2) and 10 (4) viral copies, respectively. It was also demonstrated that 'long PC R' is useful for joining together large size amplification products. ( C) 1997 Elsevier Science B.V.