O. Faktor et B. Kamensky, GENOMIC LOCALIZATION AND NUCLEOTIDE-SEQUENCE OF A LEF-8 GENE OF THE SPODOPTERA-LITTORALIS NUCLEOPOLYHEDROVIRUS, Virus genes, 15(1), 1997, pp. 9-15
A gene similar to lef-8 of the Autographa californica (Ac) nucleopolyh
edrovirus (MNPV) was identified in the Spodoptera littoralis (Spli) MN
PV, The SpliMNPV lef-8-like gene was localized on the genomic map betw
een 26.9 and 29 map units and is flanked by a chitinase gene and p47 g
ene, This gene arrangement differs from that of similar genes in the A
cMNPV genome, where the lef-8 gene is located about 62 kbp from the ch
itinase gene and about 7 kbp from the p47 gene, Sequence analyses of t
he lef-8 gene revealed an ORF of 2730 nucleotides, predicted to encode
a protein with M-r 104876, The putative protein is 60.9% identical to
the AcMNPV LEF-8 and contains an identical sequence of a conserved mo
tif of DNA-dependent RNA polymerases. Sequences downstream of the lef-
8 gene contain two sequence repeats which resemble a repeated motif of
the SpliMNPV enhancer element and other repetitive sequences from the
viral genome.