PURIFICATION OF MOUSE HI HISTONES EXPRESSED IN ESCHERICHIA-COLI

Authors
WELLMAN SE SONG YG SU DA MAMOON NM
Citation
Se. Wellman et al., PURIFICATION OF MOUSE HI HISTONES EXPRESSED IN ESCHERICHIA-COLI, Biotechnology and applied biochemistry, 26, 1997, pp. 117-123
Citations number
23
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biotechnology and applied biochemistry → ACNP
ISSN journal
08854513
Volume
26
Year of publication
1997
Part
2
Pages
117 - 123
Database
ISI
SICI code
0885-4513(1997)26:<117:POMHHE>2.0.ZU;2-Q
Abstract
We amplified the coding regions of the previously cloned HI genes HI-I , HI degrees and HIt and inserted them into the expression vector pET- IId. The synthesis of the HI histones can be induced in the appropriat e strains of bacteria, and the HI histones can be readily purified. We report detailed protocols for the purification of the expressed prote ins using combinations of ion-exchange and reverse-phase HPLC. Suffici ent amounts of each pure variant protein can be obtained for use in ph ysical studies of HI-DNA interactions.