INTERGENERIC PROTOPLAST FUSION IN SACCHAROMYCOPSIS-FIBULIGERA AND SACCHAROMYCES-CEREVISIAE

Authors
CHOI SH SUNG C OH MJ KIM CJ
Citation
Sh. Choi et al., INTERGENERIC PROTOPLAST FUSION IN SACCHAROMYCOPSIS-FIBULIGERA AND SACCHAROMYCES-CEREVISIAE, Journal of fermentation and bioengineering, 84(2), 1997, pp. 158-161
Citations number
18
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
84
Issue
2
Year of publication
1997
Pages
158 - 161
Database
ISI
SICI code
0922-338X(1997)84:2<158:IPFISA>2.0.ZU;2-#
Abstract
The amylolytic yeast Saccharomycopsis fibuligera K-33 and Saccharomyce s cerevisiae were used to breed a hybrid by protoplast fusion. S. fibu ligera K-33 produced glucoamylase and alpha-amylase. The intergeneric protoplast fusion frequency was 1.95 x 10(-4). Fusant 10-1, which enha nced alpha-amylase production, produced 3.1% (v/v) ethanol in a 10% so luble starch medium at 3 d. The amylase activity of the fusant was 2.5 times higher than that of the parental strain K-33 at 2 d.