PHYSICOCHEMICAL AND IMMUNOLOGICAL STUDIES ON THE STABILITY OF FREE AND MICROSPHERE-ENCAPSULATED TETANUS TOXOID IN-VITRO

Citation
Dkl. Xing et al., PHYSICOCHEMICAL AND IMMUNOLOGICAL STUDIES ON THE STABILITY OF FREE AND MICROSPHERE-ENCAPSULATED TETANUS TOXOID IN-VITRO, Vaccine, 14(13), 1996, pp. 1205-1213
Citations number
25
Categorie Soggetti
Immunology
Journal title
ISSN journal
0264410X
Volume
14
Issue
13
Year of publication
1996
Pages
1205 - 1213
Database
ISI
SICI code
0264-410X(1996)14:13<1205:PAISOT>2.0.ZU;2-D
Abstract
The stability of tetanus toroid released from experimental, slow-relea se microsphere vaccines was compared with that of free toroid under di fferent conditions over a 3 month period The amount of antigenicity re maining as measured by ELISA, correlated well with loss of toroid stru cture, as determined by circular dichroism and fluorescence spectrosco py, At 37 degrees C and low pH, pH 2.5 for free toxoid, or under the a cidic conditions generated by the hydrolysis of fast-release microsphe res, a gradual unfolding of the polypeptide chain was observed within the first few weeks with more rapid denaturation beyond 30 days. Copyr ight (C) 1996 Elsevier Science Ltd.