ALPHA-SUBUNITS AND BETA-SUBUNITS OF A V-TYPE MEMBRANE ATPASE IN A HYPERTHERMOPHILIC SULFUR-DEPENDENT ARCHAEUM, THERMOCOCCUS SP. KI

The genes encoding alpha- and beta-subunits of a V-type ATPase in a su lfur-dependent hyperthermophilic archaeum, Thermococcus sp. KI, were c loned and sequenced. The deduced amino acid sequences were approximate ly 60, 50 and 25% identical to those of other archaeal, eukaryotic V-t ype and E. coli F-type ATPase, respectively. Phylogenetic analysis rev ealed that Thermococcus ATPase was closely related to that of Thermus, and those of Methanosarcina and Halobacterium. (C) 1997 Elsevier Scie nce B.V.