Expression of the endoderm specific gene Endo16, was used to monitor e
ndoderm specification in developmentally arrested and in dissociated e
mbryos. beta-APN treatment halts gastrulation, however, in two species
of sea urchins Endo16 mRNA is still expressed, suggesting that specif
ication of the endodermal lineage has taken place in these development
ally arrested embryos. Endo16 mRNA is not expressed in embryos dissoci
ated at the 4-8-cell stage unless they are reassociated shortly after
the 16-cell stage. Interestingly, dissociation after the 16-cell stage
also results in a lack of Endo16 expression. Lithium is unable to res
cue Endo16 expression in these dissociated embryos. These results indi
cate that early signaling events mediated by cell-cell contact are req
uired for the initial specification and maintenance of the endoderm in
the developing embryo. (C) 1997 Elsevier Science Ireland Ltd.