CLONING, EXPRESSION, AND CRYSTALLIZATION OF A HYPERTHERMOPHILIC PROTEIN THAT IS HOMOLOGOUS TO THE EUKARYOTIC TRANSLATION INITIATION-FACTOR,EIF5A

Authors
KIM KK YOKOTA H KIM R KIM SH
Citation
Kk. Kim et al., CLONING, EXPRESSION, AND CRYSTALLIZATION OF A HYPERTHERMOPHILIC PROTEIN THAT IS HOMOLOGOUS TO THE EUKARYOTIC TRANSLATION INITIATION-FACTOR,EIF5A, Protein science, 6(10), 1997, pp. 2268-2270
Citations number
12
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
6
Issue
10
Year of publication
1997
Pages
2268 - 2270
Database
ISI
SICI code
0961-8368(1997)6:10<2268:CEACOA>2.0.ZU;2-8
Abstract
A gene coding for a protein homologous to a translation initiation fac tor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 degrees C. T he protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitan t and belong to space group P4(1)22 with unit cell dimensions a = b = 45.52 Angstrom and c = 155.59 Angstrom. These crystals diffract to at least 2.2 Angstrom resolution.