CAMPTOTHECIN AND 10-HYDROXYCAMPTOTHECIN IN CALLUS AND PLANTLETS OF CAMPTOTHECA-ACUMINATA

Authors
WIEDENFELD H FURMANOWA M ROEDER E GUZEWSKA J GUSTOWSKI W
Citation
H. Wiedenfeld et al., CAMPTOTHECIN AND 10-HYDROXYCAMPTOTHECIN IN CALLUS AND PLANTLETS OF CAMPTOTHECA-ACUMINATA, Plant cell, tissue and organ culture, 49(3), 1997, pp. 213-218
Citations number
16
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
49
Issue
3
Year of publication
1997
Pages
213 - 218
Database
ISI
SICI code
0167-6857(1997)49:3<213:CA1ICA>2.0.ZU;2-H
Abstract
The process of callus induction, organogenesis and plantlets regenerat ion of Camptotheca acuminata Decne is reported. The highest growth rat e of callus was observed on MS medium with 1 mg 1(-1) NAA, 1 mg 1(-1) kinetin and 60 gl(-1) sucrose. All tissues and organs developed in vit ro contain camptothecin and 10-hydroxycamptothecin. The presence of 10 -hydroxycamptothecin in shoots and callus of Camptotheca acuminata Dec ne is reported for the first time. The alkaloids were detected and ide ntified using HPLC methods.