DIRECT CLONING OF THE BRASSICA S-LOCUS BY USING A P1-DERIVED ARTIFICIAL CHROMOSOME (PAC) VECTOR

Authors
SUZUKI G WATANABE M TORIYAMA K ISOGAI A HINATA K
Citation
G. Suzuki et al., DIRECT CLONING OF THE BRASSICA S-LOCUS BY USING A P1-DERIVED ARTIFICIAL CHROMOSOME (PAC) VECTOR, Gene, 199(1-2), 1997, pp. 133-137
Citations number
20
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
199
Issue
1-2
Year of publication
1997
Pages
133 - 137
Database
ISI
SICI code
0378-1119(1997)199:1-2<133:DCOTBS>2.0.ZU;2-M
Abstract
Self-incompatibility of Brassica is regulated by the S locus, which co ntains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S-9 ha plotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the Pl-derived artificial chromosome (PAC) vector. T he utility of the direct cloning method is discussed in this study. (C ) 1997 Elsevier Science B.V.