NEW STAINING METHODS FOR SPERM EVALUATION ESTIMATED BY MICROSCOPY ANDFLOW-CYTOMETRY

Citation
M. Magistrini et al., NEW STAINING METHODS FOR SPERM EVALUATION ESTIMATED BY MICROSCOPY ANDFLOW-CYTOMETRY, Theriogenology, 48(7), 1997, pp. 1229-1235
Citations number
32
Categorie Soggetti
Veterinary Sciences
Journal title
ISSN journal
0093691X
Volume
48
Issue
7
Year of publication
1997
Pages
1229 - 1235
Database
ISI
SICI code
0093-691X(1997)48:7<1229:NSMFSE>2.0.ZU;2-M
Abstract
New staining methods and automated instruments are now available to ev aluate the sperm cell in vitro. Individual compartments of the sperm c ell, such as the nucleus and the plasma and acrosomal membranes, may b e investigated, as well as the cell, function as shown by mitochondria activity and capacitation. Various probes are used and they can be an alyzed by direct light or fluorescent microscopy or by flow cytometry. The automated instruments allow objective and accurate analysis and q uantification as well as the ability to evaluate large population of c ells in a shorter time, thus providing accurate evaluation of sperm qu ality. However, before these test can be recommended for routine clini cal and investigational use, in the stallion, they need to be confirme d on a larger number of stallions and their correlation with tradition al semen parameters and with stallion fertility has to be demonstrated . (C) 1997 by Elsevier Science Inc.