Threats to imperiled freshwater fauna in the U.S. were assessed throug
h an experts survey addressing anthropogenic stressors and their sourc
es. Specifically, causes of historic declines and current limits to re
covery were identified for 135 imperiled freshwater species of fishes,
crayfishes, dragonflies and damselflies, mussels, and amphibians. The
survey was designed to identify threats with sufficient specificity t
o inform resource managers and regulators faced with translating infor
mation about predominant biological threats into specific, responsive
actions. The findings point to altered sediment loads and nutrient inp
uts from agricultural nonpoint pollution; interference from exotic spe
cies; and altered hydrologic regimes associated with impoundment opera
tions as the three leading threats nationwide, accompanied by many les
ser but still significant threats. Variations in threats among regions
nad among taxa were also evident. Eastern species are most commonly a
ffected by altered sediment loads from agricultural activities, wherea
s exotic species, habitat removal/damage, and altered hydrologic regim
es predominate in the West. Altered sediment loading from agricultural
activities and exotic species are dominant problems for both eastern
mussels and fishes. However, eastern fishes also appear to be sufferin
g from municipal nonpoint pollution (nutrients and sediments), whereas
eastern mussels appear to be more severely affected by altered nutrie
nt impacts from hydroelectric impoundments and agricultural runoff. Ou
r findings suggest that control of nonpoint source pollution associate
d with agriculture activities should be a very high priority for agric
ultural producers and governmental support programs. Additionally, the
large number of hydropower dams in the U.S. subject to federal re-lic
ensing in coming years suggests a significant opportunity to restore n
atural hydrologic regimes in the affected rivers.