Ca. Praul et Wd. Taylor, RESPONSES OF HALOBACTERIUM-HALOBIUM AND SULFOLOBUS-SOLFATARICUS TO HYDROGEN-PEROXIDE AND N-METHYL-N-NITROSOGUANIDINE EXPOSURE, Microbiological research, 152(3), 1997, pp. 257-260
We have examined the capacity of Halobacterium halobium and Sulfolobus
solfataricus to withstand the lethal effects of hydrogen peroxide and
N-methyl-N-nitrosoguanidine. We tested a variety of pretreatment regi
mens with both mutagens and all failed to elicit an inducible response
to the lethal effects of these compounds in either organism. We have
observed AP endonuclease activity in protein extracts from both organi
sms. In addition, S. solfataricus extracts contain activities that rem
ove 3-methyl-adenine and O-6-methyl-guanine from methylated DNA. This
is the first report of these DNA repair activities in any member of th
e Archaea.