CONCOMITANT AND HORMONALLY REGULATED EXPRESSION OF TRP GENES IN BOVINE AORTIC ENDOTHELIAL-CELLS

Recent Findings have suggested that the vertebrate trp family of chann el proteins is the structural basis for Ca2+ influx through the capaci tative calcium entry (CCE) pathway. We have discerned, in bovine aorti c endothelial cells, the concomitant expression of four such vertebrat e genes: trp-l (two splice variants), trp-3, trp-4 and trp-5. Exogenou s hormones rendered dynamic effects on the transcript levels of these genes, Most notably, beta-estradiol significantly down-regulated trp-4 while trans-retinoic acid dramatically up-regulated trp-5; yet these hormones rendered little change in CCE, These findings suggest that th e extent of a given trp channel's participation in CCE is not reflecte d in alterations of its transcript level. (C) 1997 Federation of Europ ean Biochemical Societies.