PRELIMINARY NMR-STUDIES OF THERMUS-THERMOPHILUS RIBOSOMAL-PROTEIN S19OVERPRODUCED IN ESCHERICHIA-COLI

Authors
DAVYDOVA NL RAK AV GRYAZNOVA OI LILJAS A JONSSON BH BERGLUND H HARD T GARBER MB
Citation
Nl. Davydova et al., PRELIMINARY NMR-STUDIES OF THERMUS-THERMOPHILUS RIBOSOMAL-PROTEIN S19OVERPRODUCED IN ESCHERICHIA-COLI, FEBS letters, 415(2), 1997, pp. 155-159
Citations number
31
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
415
Issue
2
Year of publication
1997
Pages
155 - 159
Database
ISI
SICI code
0014-5793(1997)415:2<155:PNOTRS>2.0.ZU;2-Q
Abstract
The gene for the ribosomal protein S19 from Thermus thermophilus was c loned, sequenced and overexpressed in Escherichia coli. A simple proce dure for isolating the recombinant protein was developed. Preliminary NMR studies revealed a high content of alpha-helical secondary structu re in the protein. (C) 1997 Federation of European Biochemical Societi es.