Surface energy characteristics of a solid surface influence the extent
and rate of protein adsorption, However, critical surface tension det
ermined by contact angle measurements, cannot be used to predict the e
xtent or rare of milk whey protein fouling onto a particular surface,
Radioisotopic iodination of alpha-la and beta-lg was used to show that
protein adsorption onto a surface is dependent on protein concentrati
on and temperature. Multilayer adsorption was observed with a single p
rotein solution and with sequential exposure to the two whey proteins.